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Surface here is educational only; do not use without medical supervision. Our editorial verdict is SKIP-FOR-NOW — current cost / risk / redundancy puts it below the line.

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Neboglamine

Well Researched

Italian-origin (Rottapharm) glutamic-acid-derived positive allosteric modulator at an allosteric site of the NMDA glycine co-agonist…

Aliases (5)
Nebostinel · CR-2249 · CR2249 · XY-2401 · (S)-4-amino-5-[(4,4-dimethylcyclohexyl)amino]-5-oxo-pentanoic acid
TYPICAL DOSE
25-50 mg
Daily
ROUTE
Oral (tablet)
Oral
CYCLE
Not enough data to recommend a defensible cycle
As prescribed
STORAGE
Room temp; original container
Room temp

Overview

What is Neboglamine?

Neboglamine (CR-2249) is a positive allosteric modulator of the NMDA receptor glycine binding site, originally developed by Rotta Pharmaceuticals as an investigational antipsychotic and cognitive enhancer for schizophrenia.

Key Benefits

Investigated for treating negative and cognitive symptoms of schizophrenia, where NMDA hypofunction is implicated. Reported pro-cognitive effects on memory and attention without the receptor desensitization typical of orthosteric NMDA agonists.

Mechanism of Action

Selective positive allosteric modulator at the glycine B (strychnine-insensitive) site of the NMDA receptor, enhancing glutamate-driven receptor activation only when glutamate is bound, providing activity-dependent NMDA potentiation.

Pharmacokinetics

·
PeakHalf-life
Approximate curve — visual aid only, not data-precise PK
Brand options4 known
NebostinelCR-2249CR2249XY-2401

Status"Investigational worldwide. Not scheduled. Not approved by FDA, EMA, PMDA. Highest reached: Phase 2 (schizophrenia, cocaine dependence) under Rottapharm SpA — last public registry update 2010; no Phase 3 program; status appears stalled rather than formally discontinued."

Research Indications

Most Effective

Facilitates glycine action

increases [³H]MK-801 binding (an open-channel marker of NMDA activation) in a concentration-dependent manner, with positive cooperativity…

Effective

Reverses kynurenic acid antagonism

kynurenic acid is the endogenous tryptophan-pathway metabolite that *antagonizes* the glycine site (and the α7 nicotinic receptor), and K…

Investigational

Activity-dependent

PAM behavior means it requires endogenous glycine to do anything. With no glycine around, neboglamine alone does nothing at the receptor.…

Investigational

In rats, neboglamine increased FLI (Fos-like immunoreactivity) in prefrontal cortex similarly to haloperidol and clozapine

the antipsychotic-overlap behavioral signal that motivated the schizophrenia program (Pubmed 20045056, Drago/Caccia/Spada 2010).

Investigational

Weak NRI activity at supratherapeutic exposures (>100 mg)

at clinical doses (25-75 mg) this is not the dominant mechanism; at higher research-chem doses it would contribute and add a stimulant-fl…

Investigational

Plasma half-life ~4 hours

reported short, requiring multi-daily dosing for sustained NMDA-tone elevation.

Research Protocols

Disclaimer: These are commonly discussed research protocols and not medical advice.

Goal:Phase 1
Dose:200 mg appears in some early-pharmacology references but does not establish a clinical efficacy dose
Frequency:
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Cycle:
Goal:Format
Dose:1 g of HCl powder for ~$60)
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Goal:Half-life implication
Dose:
Frequency:AM
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Goal:Track
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Goal:Hard exclusion: do not run concurrent with TAK-653, agmatine, or memantine
Dose:
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Goal:Combat-sport training-day rule
Dose:
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Peptide Interactions

D-serine, glycine, sarcosine
Synergistic

same direction, but the whole point of a PAM over an agonist is that you don't *need* to load the agonist binding partner. Stacking would be redundant and wo…

Modafinil
Synergistic

orthogonal mechanism (DAT/NET inhibition + histaminergic + orexinergic). No formal data. Sequential layering (establish modafinil baseline first) is the only…

TAK-653
Avoid

the encyclopedia's pre-existing flag stands. Two unproven glutamate-system amplifiers (AMPA PAM + NMDA glycine PAM) at the same time multiplies unknowns and …

Memantine
Avoid

directly opposing on the NMDA axis (memantine = uncompetitive NMDA channel blocker; neboglamine = NMDA potentiator at the glycine co-agonist site). They don'…

Agmatine
Avoid

agmatine is a modest GluN2B-preferring NMDA antagonist + nNOS modulator. Same opposing-direction logic as memantine, weaker in absolute effect. For trial cle…

Ketamine, esketamine, dextromethorphan (high-dose), nitrous oxide, phencyclidine-class
Avoid

all NMDA antagonists; opposing direction; do not run together.

D-cycloserine, D-serine, glycine high-dose, sarcosine, bitopertin/iclepertin/luvadaxistat
Avoid

same-direction glutamate-system enhancers via different mechanisms. Stacking is mechanistically pointless and additively risky.

Tramadol, bupropion, pre-workout caffeine spikes >300 mg, designer cathinones, MDMA
Avoid

additive cortical excitability / theoretical seizure-threshold issues. Not relevant for the canonical stack but worth flagging.

First-gen AMPAkines (CX-516, sunifiram, unifiram, racetams in glutamate-system framing)
Avoid

mechanism overlap; no point and adds risk.

Flag
Compatible

NOW Foods Glycine 3 g/day in the canonical stack is a borderline case — at oral 3 g, plasma glycine rises modestly, but synaptic glycine is heavily reuptake-…

Quality Indicators

Pharmacy-dispensed, intact packaging

Prescription tablets in original sealed packaging from a licensed pharmacy.

!

Generic vs branded

Generics are usually fine but bioavailability can vary slightly; track if you switch.

Unbranded blister or counterfeit risk

Counterfeit pharmaceuticals are a known issue; verify pharmacy and lot if buying internationally.

What to Expect

  • Day 1
    PK-driven acute peak per administration. Verify dose tolerated.
  • Week 1
    Steady-state reached for most daily-dosed pharma.
  • Week 2-4
    Therapeutic effect established; titration window if needed.
  • Long-term
    Periodic monitoring per drug class (labs, BP, ECG as applicable).

Side Effects & Safety

  • Common (>10% in trials): Not reported above placebo at clinical doses in published Phase 1 summaries. Granular AE rates are not in the public domain — interpret with caution.

  • Less common (1-10%): Headache, mild nausea, occasional dizziness reported at higher doses in unsourced biohacker accounts. Insomnia possible if dosed late given the NRI activity at higher exposures.

  • Rare-serious / theoretical (<1% but worth knowing):

    • Excitotoxicity / seizure threshold: theoretical concern shared with all glycine-site enhancers. Activity-dependent PAM mechanism should limit risk vs direct agonists, but no human seizure data exists in healthy long-term use. Combined with subconcussive impact load (the canonical archetype), this is the most material risk.
    • Pro-psychotic in vulnerable individuals: NMDA agonism/PAM action can theoretically destabilize people with prodromal psychosis or strong family-history risk. Schizophrenia trial population was already-diagnosed and on adjunctive antipsychotic; healthy-adult risk window is not characterized.
    • Sleep disruption: NRI activity at higher doses + cortical-excitability angle = unfavorable for late-evening dosing.
    • Long-term safety: zero data beyond Phase 2 short-duration windows. Chronic NMDA-glycine-site potentiation has no human safety record beyond ~weeks-to-months in trial-grade settings. Receptor-system adaptation, spine-density changes, plasticity rebound on discontinuation — all theoretical and untested.
    • Drug development "stall" implication: when a Phase 2 program goes quiet for 10+ years without formal discontinuation announcement, the most parsimonious explanation is a failed efficacy or unfavorable risk/benefit signal that the sponsor chose not to publish. This is not provable from public data, but it is a real Bayesian concern.
  • Specific watch periods for any pilot:

    • Days 1-3 (single-dose tolerability): acute headache, nausea, dizziness, any aura-flavored phenomena, BP spike, sleep onset.
    • Days 4-10 (chronic tone): mood drift (low or high), anxiety, irritability, sleep architecture changes.
    • Discontinuation Days 1-7: rebound watch — no published taper data, no withdrawal syndrome characterized, but plasticity-system rebound is the theoretical concern.

References

Neboglamine — Wikipedia

en.wikipedia.org · 2015

basic mechanism + Phase 2 status snapshot (last meaningful update 2015).

View Study

Synapse Patsnap — Neboglamine drug page

synapse.patsnap.com

registry-tier development status; lists schizophrenia as currently active, Alzheimer's/MDD/cocaine as inactive.

View Study

NCATS Inxight Drugs — Neboglamine

drugs.ncats.io

UNII 12EA34U5B8; FDA-side database entry confirming sponsor/mechanism.

View Study

Lanza et al., 1996 — *Pharmacol Biochem Behav* — CR 2249 memory enhancer

pubmed.ncbi.nlm.nih.gov · 1996

passive avoidance learning rodent paradigm; original cognitive-enhancement paper.

View Study

Lanza et al., 1997 — *Neuropharmacology* — characterization of glycine-site PAM mechanism

pubmed.ncbi.nlm.nih.gov · 1997

kynurenate reversal, MK-801 binding cooperativity; foundational pharmacology.

View Study
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