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Overview
What is TB-500?
TB-500 is a synthetic 7-amino acid fragment (Ac-LKKTETQ) corresponding to the active actin-binding region (positions 17-23) of thymosin beta-4. Originally developed for veterinary use in horses, this fragment retains tissue repair and anti-inflammatory properties while being more stable than the full peptide. Banned by WADA for its performance-enhancing effects
Key Benefits
Superior systemic tissue repair, accelerated healing of muscles, tendons, ligaments, and organs. Enhanced cell migration and angiogenesis for comprehensive regenerative effects.
Mechanism of Action
Injectable TB-500 provides systemic distribution for whole-body tissue repair. Acts as the principal actin-sequestering protein, regulating cell migration, promoting angiogenesis, reducing inflammation, and activating stem cell differentiation for accelerated healing.
Molecular Information
Weight
4,963.44 Da
Length
43 amino acids
Type
Thymosin fragment
Amino Acid Sequence:
Ac-Ser-Asp-Lys-Pro-Asp-Met-Ala-Glu-Ile-Glu-Lys-Phe-Asp-Lys-Ser-Lys-Leu-Lys-Lys-Thr-Glu-Thr-Gln-Glu-Lys-Asn-Pro-Leu-Pro-Ser-Lys-Glu-Thr-Ile-Glu-Gln-Glu-Lys-Gln-Ala-Gly-Glu-Ser
* N-terminal acetylation for enhanced stability
Pharmacokinetics
Research Indications
Muscle Regeneration
Accelerated healing of muscle fibers, reduced recovery time from muscle injuries, and enhanced muscle tissue repair through improved cell migration and differentiation.
Tendon & Ligament Healing
Promotes connective tissue repair, reduces scar formation, and improves biomechanical properties of healing tendons and ligaments.
Wound Healing
Enhanced dermal wound closure, improved angiogenesis, and reduced inflammation in both acute and chronic wound healing scenarios.
Research Protocols
Disclaimer
TB-500 is commonly reported with a loading phase (4-6 weeks at 2-2.5mg 2x/week) followed by a maintenance phase (2-4mg every 1-2 weeks). Tapering as symptoms improve is often discussed rather than abrupt discontinuation. This is not medical advice. Consult a healthcare provider before use.
Timing
TB-500 timing is flexible and doesn't require empty stomach. For localized injuries, inject as close to the injury site as possible. For systemic effects, standard subcutaneous injection sites work well.
Peptide Interactions
How to Reconstitute
Important
Always use bacteriostatic water (BAC). Sterile technique is essential.
Clean work area and hands thoroughly with alcohol
Calculate required BAC water volume using calculator below
Draw calculated BAC water slowly into syringe
Inject BAC water slowly down the side of vial (never directly onto powder)
Gently swirl vial until powder completely dissolves (never shake vigorously)
Store reconstituted solution in refrigerator and use within 28 days
Quality Indicators
White Lyophilized Powder
TB-500 should appear as a white to off-white, fine powder that is evenly distributed in the vial. Proper lyophilization creates a light, fluffy texture.
Clear Solution After Reconstitution
When properly reconstituted with BAC water, TB-500 should form a completely clear, colorless solution with no visible particles or cloudiness.
Proper Vacuum Seal
Vial should have intact vacuum seal with no cracks or damage. The rubber stopper should be secure and show no signs of contamination.
Slight Powder Clumping
Minor clumping during shipping is acceptable if it dissolves completely with gentle swirling. Avoid shaking vigorously.
Colored or Crystalline Powder
Any discoloration (yellow, brown) or crystalline appearance may indicate degradation or contamination. Should appear as fine white powder only.
Persistent Cloudiness
If solution remains cloudy, has floating particles, or shows precipitation after reconstitution, the peptide may be degraded or contaminated.
What to Expect
- Week 1-2: Reduced inflammation and improved tissue sensation
- Week 2-4: Accelerated healing rate and improved tissue quality
- Week 4-8: Maximum tissue repair and regenerative benefits
- Week 6-12: Continued improvement in tissue strength and function
- Side effects: Generally minimal, possible injection site reactions
- Most effective for: Muscle, tendon, ligament, and soft tissue injuries
Side Effects & Safety
- Use sterile injection technique to prevent infection
- Mild injection site reactions are normal and temporary
- Consult healthcare provider if on immunosuppressive medications
- Not recommended during active cancer treatment
- Avoid during pregnancy or breastfeeding
- WADA prohibited - not for competitive athletes
References
Cardiac Tissue Regeneration (2016)
Pilot study showed TB-500 pre-treated endothelial progenitor cell transplantation improved left ventricular function by >50% in post-MI patients.
Muscle Fiber Regeneration (2015)
TB-500 improved skeletal muscle fiber regeneration in dystrophin-deficient mice, showing potential for muscular dystrophy treatment.
Corneal Healing Research (2014)
Topical TB-500 eye drops significantly improved signs and symptoms of moderate to severe dry eye with lasting effects beyond treatment period.
Quick Start Guide
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